Figure 6.

Fluorescent all-in-focus imaging for intravital surgery. During intraoperative surgery, the use of all-in-focus imaging in fluorescent mode, would enable the detection of extended tumors and small lesions within tissue margins (d,f). Panel (c,e) demonstrates the impossibility of identifying small lesions or metastases when the images are not in focus. If small tumoral masses lie at different depths, scanning along the axis of the microscope lens is necessary for accurate detection. Panels (a–b) show the location of the tumors within the mouse in white light and fluorescent mode respectively