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. Author manuscript; available in PMC: 2014 Apr 2.
Published in final edited form as: Angew Chem Int Ed Engl. 2013 Feb 28;52(14):3931–3934. doi: 10.1002/anie.201209303

Figure 1. FLIM to detect phosphorylation-dependent fluorophore lifetime shifts for biosensors in intact, live cells.

Figure 1

A fluorophore labeled peptide substrate is delivered to live cells via a cell-penetrating peptide. Phosphorylation of the substrate results in observation of increased fluorophore lifetime via FLIM.