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Kinetic data associated with various ecDHFR constructs. ND = Not determined.
| Construct | khyd at pH 17.0 (s−1)3 | kFRET at pH 17.0 (s−1) | kFRET pH effect | kFRET KIE |
|---|---|---|---|---|
| WT1 | 220 ± 10 | NA | ND | ND |
| A | 186 ± 40 | 164 ± 40 | No | (0.9±0.2) |
| A L54I | 2.5 ± 1 | 27 ± 6 | No | ND |
| B | 172 ± 48 | 156 ± 42 | No | ND |
| C | 116 ± 30 | 122 ± 40 | ND | (1.1±0.1) |
| D | 190 ± 20 | 2.0 ± 0.5 | No | (1.1±0.2) |
| E2 | 210 ± 40 | 282 ± 40 | No | (1.2±0.1) |
| E2 G121V | 3.5 ± 1 | 154 ± 40 | ND | ND |
