Table 2.

Temperatures at which the maximum MRI signal enhancement rate occurred in Mn-LTSL samples suspended in saline

concentration	temperature (°C)	peak direction	T-test vs Tm *
0.06mM	42.5 ± 0.3 °C	positive	P = 0.26
0.12mM	43.1 ± 0.5 °C	positive	P = 0.79
0.25mM	43.1 ± 1.1 °C	positive	P = 0.89
0.5mM	43.6 ± 0.6 °C	positive	P = 0.31
1.0mM	-†	-	-
2.0mM	43.4 ± 0.7 °C	negative	P = 0.48
4.0mM	44.6 ± 0.6 °C	negative	P = 0.08
0.06mM – 0.5mM	43.1 ± 0.3 °C	positive	P = 0.67
All samples	43.3± 0.3 °C	not applicable	P = 0.15

Initial encapsulated MnSO₄ concentration: 63mM. Four replicates were performed for each concentration.

Error is given as the standard error of the mean.

Lipid composition: DPPC:MSPC:DSPE-PEG2000 (mol %) = 85.0 : 9.8 : 5.2.

^*Using a two-tailed heteroscedastic t-test, it was tested if the temperature corresponding to the peak signal enhancement rate on T₁ weighted images was significantly different from the phase transition temperature of the liposome bilayer in saline measured with DSC (T_m = 42.92 ± 0.02 °C).

^†No peak in signal enhancement rate was observed for the 1.0mM samples.