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. Author manuscript; available in PMC: 2013 Sep 23.
Published in final edited form as: Transplantation. 2006 Dec 15;82(11):1510–1517. doi: 10.1097/01.tp.0000243168.53126.d2

FIGURE 3.

FIGURE 3

Immunohistology evaluation of islet allografts. Serial kidney sections were isolated 12 days after islet allograft transplantation. Sections were evaluated and the number of CD8-positive cells in five consecutive high-power fields were counted and expressed as cells per high-power field (mean±SD). Untreated recipients show dense tissue infiltration surrounding and invading the islets with a predominance of CD8+ T cells (A). Recipients treated with CTLA4/Fc show a decreased interstitial lymphocytic infiltrate with preservation of the tissue structure (B). Recipient mice receiving IL-15 mutant/Fcγ2a present moderate CD8+ T cell infiltrates (C). Treatment with IL-15 mutant/Fcγ2a prevents CD8 T cell infiltrates (CD8-positive cells IL-15 mutant/Fcγ2a: 50±6 vs. untreated: 181±10: P<0,0001; CD8-positive cells IL-15mutant/ Fcγ2a: 50±6 vs. CTLA4/Fc: 85±9; P<0.002). As expected, CD4+ T cells were undetectable in the grafts (D–F) (Cryostat sections, hematoxylin counterstain, × 100 magnification). These are representative sections from three recipients from each treatment group.