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. 2013 Sep 23;4:282. doi: 10.3389/fmicb.2013.00282

Figure 1.

Figure 1

In-frame deletion of genes involved in starch degradation and phosphate hydrolysis from the E. coli EPI300-T1R expression host. (A) Starch degradation pathway. Deleted genes have been crossed out. (B) Phosphatase catalyzed reactions that were knocked out. (C) Agarose gel electorophoresis of PCR amplicons before and after gene deletion. For each gene, the first well is PCR negative control, second is wild type E. coli EPI300-T1R and third is E. coli EPI300-T1R after gene deletion (EC 3.2.1.3 α-glucosidase; EC 3.1.3.1 alkaline phosphatase; EC 3.6.1.11 phosphoanhydride phosphohydrolase; EC 3.2.1.1 cytoplasmic or periplasmic α-amylase; EC EC 3.2.1.20 maltodextrin glucosidase; EC 3.1.3.2 acid phosphatase).