Figure 2.

Screening workflow combining automated colony picking and liquid handling. In solid media assays clones are individually gridded on agar trays containing screening media. In solution-based assays a two-step clone pooling approach is employed. First, 16 × 96-well plates are combined on one 96-well substrate plate so that each well contains 16 different clones. After identification of positive wells, the 16 clones pooled in the respective well are expanded for individual screening. By repeating the pooling and gridding eight times, 12,160 clones can be screened simultaneously for 14 different activities in a total of 170,240 reactions.