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. 2013 Sep 23;4:282. doi: 10.3389/fmicb.2013.00282

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Copyright © 2013 Nyyssönen, Tran, Karaoz, Weihe, Hadi, Martiny, Martiny and Brodie.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Emergent self-organizing map (ESOM) of the sequences from the positive clones. The map was generated based on tetranucleotide frequencies calculated from contigs fragmented to 2 Kbp. In ESOM, sequences with similar tetranucleotide frequencies cluster together in multidimensional space. Here, these clusters, i.e., bins, are indicated with numbers with phylogenetic affiliations as follows: (1) Actinobacteria, (2) Fungi, (3) Bacteroidetes, (4) Bacteroidetes, (5) Bacteroidetes, (6) γ-Proteobacterial, (7) α-Proteobacteria, (8) β-Proteobacteria, (9) γ-Proteobacteria, and (10) α-Proteobacteria. (B) ESOM with sequence fragments colored by the sampling time point that leaf litter was collected for fosmid library construction (Green: December 2010; Blue: February 2011; Red: March 2012).