Table 3.
Number of positive assays observed during the screening of leaf litter metagenomic libraries with the functional screening platform developed in this study.
| Library | Dec10_01XX | Dec10_08XX | Feb11_01XX | Feb11_08XX | Feb11_16XN | Mar12_01XX | Mar12_08XX |
|---|---|---|---|---|---|---|---|
| Number of clones | 21,755 | 18,540 | 10,830 | 15,105 | 26,818 | 25,080 | 25,080 |
| AZCL-HE-cellulose | – | 1 | – | – | 4 | – | – |
| 4-MUB-β-D-cellobiose | 12 | 23 | 13 | 9 | 31 | 6 | 6 |
| PNP-β-D-glucopyranoside | 6 | 11 | 9 | 6 | 14 | 4 | 1 |
| AZCL-Xylan | 5 | 5 | 7 | 6 | 6 | 2 | 1 |
| 4-MUB-β-D-Xyloside | 24 | 19 | 20 | 11 | 31 | 14 | 8 |
| Starch azure | 2 | 1 | 2 | – | 1 | 1 | – |
| 4-MUB-α-D-glucoside | 4 | 4 | – | 2 | 3 | – | – |
| Chitin azure | – | – | – | – | – | – | – |
| 4-MUB-β-N-acetylglucosamine | 8 | 16 | 7 | 15 | 19 | 2 | 4 |
| L-dihydroxyphenylalanine | – | – | – | – | – | – | – |
| Syringaldazine | – | – | – | – | – | – | – |
| 5-Bromo-4-chloro-3-indolyl phosphate | 9 | 10 | 4 | 3 | 7 | – | 1 |
| Skim milk | – | 1 | 1 | – | – | – | 2 |