Table 1.
Colony Forming Unit-Fibroblastic Efficiency (%) of Cells Derived from Umbilical Cord, Amniotic Membrane, and Bone Marrow
| Donor | UC | AM | BM |
|---|---|---|---|
| 1 | 0.6±0.2 | <0.03a | n.d. |
| 2 | 0.9±0.2 | 1.3±0.2 | n.d. |
| 3 | 1.8±0.4 | >33.3b | n.d. |
| 4 | 0.6±0.1 | 0.6±0.2 | n.d. |
| 5 | 0.2±0.1 | 0.5±0.1 | n.d. |
| 6 | n.d. | n.d. | 0.008±0.001 |
| 7 | n.d. | n.d. | 0.005±0.001 |
The closest approximation to estimate the frequency of MSCs derives from the CFU-F efficiency assay. CFU-F efficiency (%) represents the ratio of number of cells forming colonies under clonal conditions and number of cells seeded directly after isolation of cells from UC, AM, or BM. Data show mean values±standard deviations (n=6).
AM-derived cells from donor 1 did not form colonies at the highest cell density of 3,000 cells/cm2, that is, CFU-F efficiency was less than 1/3,000 (<0.03%).
AM-derived cells from donor 3 formed more than 100 colonies at the lowest cell density of 300 cells/cm2, that is, CFU-F efficiency was higher than 100/300 (>33.3%).
CFU-F, colony forming unit-fibroblastic; AM, amniotic membrane; BM, bone marrow; UC, umbilical cord; n.d., not determined; MSCs, mesenchymal stromal cells.