FIG. 1.

Inflammation of murine LNs following administration of influenza. (a) Size increase in the lung-draining mediastinal lymph node (MLN) at 0, 24, 48, and 72 h following infection with influenza strain A/H1N1 Puerto Rico 8 (PR8). (b) Weight increases in popliteal LNs (PLNs) collected from mice injected SC in the footpad with PBS or UV-inactivated PR8 and in MLNs from mice administered PBS or PR8 intratracheally. Both PLNs and MLNs were collected at 72 h post-injection/infection. (c) Comparison of confocal and MP imaging of whole-mount LN tissue. PLNs from CD11c-eYFP reporter mice were isolated and imaged at depths from 0–200 μm to demonstrate comparative tissue penetration. A color version of this figure is available in the online article at www.liebertpub.com/lrb