FIG. 2.

Optical clearing of whole LNs. (a) Confocal micrograph of optically cleared PLN. mAb specific for the subcapsular sinus (anti-CD169, shown in green) and medulla (anti-F4/80; shown in blue) were injected SC 4h prior to LN harvest. (b) Confocal micrograph of optically cleared PLN, illustrating the complexity of the vascular and follicular architecture within the PLN. C57BL/6 mouse was injected IV with antibodies specific for blood vessels (anti-CD31; shown in red) 4h prior to LN harvest and was injected IV with antibodies specific for follicular dendritic cells (anti-8C12; shown in white) 24h prior to LN harvest. (c) Cartoon of approximate tissue penetration depths achievable through different imaging approaches. A color version of this figure is available in the online article at www.liebertpub.com/lrb