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Serial imaging and whole LN reconstruction of the PLN. (a) Serial images used for reconstruction of in vivo labeled PLN. CD11c-eYFP mice were pre-injected SC with antibodies specific for CD169 (green) and CD35 (red), and PLNs were harvested 1 h after PBS injection into the footpad. PLN was sectioned (50 μm cryosections) and imaged for reconstruction. (b) Reconstructed image of a PLN from (a).
