FIG. 4.

Automatic tiling and whole LN reconstruction approach.Thick tissue sections, either labeled in vivo with antibodies injected prior to LN harvest or labeled post-sectioning, are imaged as individual fields of view, or “tiles.” The tiles are then stitched together, resulting in an xy panorama for each tissue section. The sections are then compiled in the z direction, resulting in a fully reconstructed LN, ready for subsequent examination and analysis. A color version of this figure is available in the online article at www.liebertpub.com/lrb