Fig. 10.
Fractional inhibition of native and specifically mutated rat Nav1.4 sodium channels expressed in Xenopus oocytes by SCI insecticides. The extent of inhibition was determined following perfusion with insecticide (10 µM) as described in the legend to Fig. 9. The Nav1.4/V787A and Nav1.4/V787C variants were assayed using the pulse protocol described in Fig. 9 for Nav1.4 channels. Asterisks indicate inhibition of mutated channels that is significantly different from that measured for the same compound assayed against native Nav1.4 channels. The extent of inhibition (top) is correlated with the relative hydrophobicity of amino acid residues (bottom). The amino acids at position 787 in the four channel variants employed are shown in boldface type. The figure is drawn from the data of von Stein and Soderlund [22].