Table 1.

Characterisation of monooxygenase activity from Stenotrophomonas maltophilia PM102.

Conditions for enzyme activity	Enzyme parameters	Specific activity (U/mg) (4 hrs)
Metal cofactors (1 mM)	Cu, Fe, and Mn	90.6, 25, and 11.2
Optimum metal cofactor	Cu
Range of copper concentration tested	0.05 mM–5 Mm
Optimum Cu concentration	0.7 mM	96.87
Range of NADH concentration	0.01 mM–0.2 mM
Optimum NADH concentration	0.05 mM	140.625
Range of temperature	4°C–42°C
Optimum temperature	36°C	103.13
Range of pH	3–10
Optimum pH	5 and 8	100 and 120
Substrates	TCE, benzene, toluene, chloroform, hexane, and petroleum benzine	87.5, 103.75, 31.87, 40.63, 13.75, and 11.25
Optimum substrate	Benzene	103.75
Rate when benzene is the substrate	V max⁡ Benzene	340 U/mg/min
Rate when TCE is the substrate	V max⁡ TCE	170 U/mg/min
K m when benzene is the substrate	K m Benzene	3.8 mM
K m when TCE is the substrate	K m TCE	2.1 mM