Table 1.
Characterisation of monooxygenase activity from Stenotrophomonas maltophilia PM102.
| Conditions for enzyme activity | Enzyme parameters | Specific activity (U/mg) (4 hrs) |
|---|---|---|
| Metal cofactors (1 mM) | Cu, Fe, and Mn | 90.6, 25, and 11.2 |
| Optimum metal cofactor | Cu | |
| Range of copper concentration tested | 0.05 mM–5 Mm | |
| Optimum Cu concentration | 0.7 mM | 96.87 |
| Range of NADH concentration | 0.01 mM–0.2 mM | |
| Optimum NADH concentration | 0.05 mM | 140.625 |
| Range of temperature | 4°C–42°C | |
| Optimum temperature | 36°C | 103.13 |
| Range of pH | 3–10 | |
| Optimum pH | 5 and 8 | 100 and 120 |
| Substrates | TCE, benzene, toluene, chloroform, hexane, and petroleum benzine | 87.5, 103.75, 31.87, 40.63, 13.75, and 11.25 |
| Optimum substrate | Benzene | 103.75 |
| Rate when benzene is the substrate | V max Benzene | 340 U/mg/min |
| Rate when TCE is the substrate | V max TCE | 170 U/mg/min |
| K m when benzene is the substrate | K m Benzene | 3.8 mM |
| K m when TCE is the substrate | K m TCE | 2.1 mM |