TABLE I.
Primer sequences for cloning the genomic region upstream of TNFRSF13C.
−2.5 kb +KpnI 5′ | 5′ GAATCA GGTACC TATGTGGGTGGCAGGGTTATGGC 3′ |
−2.0 kb +KpnI 5′ | 5′ GAATCA GGTACC GCTCAGAATCTCCCTCCTACCC 3′ |
−1.5 kb +KpnI 5′ | 5′ GAATCA GGTACC AGATAGACAGGAGGGGCCCGC 3′ |
−1.0 kb +KpnI 5′ | 5′ GAATCA GGTACC CTGGGCACTGTGGCTTCACACC 3′ |
−0.5 kb +KpnI 5′ | 5′ GAATCA GGTACC ACACCTCCCAGCACCCAGCAG 3′ |
−0 kb +HindIII 3′ | 5′ GAATCA AAGCTT CGACGCCGCCGCACAAGCTGC 3′ |
Primers used to clone the region upstream of TNFRSF13C for the promoter reporter constructs. The numbering in the name of each probe indicates its approximate position relative to the TNFRSF13C transcriptional start site (TSS) followed by the restriction enzyme cleavage site added to the sequence. The added restriction site is underlined and separated from the neighboring nucleotides by spaces.