TABLE III.
EMSA probes.
| Region of Probe (Relative to TSS) | Direction | Sequence, 5′ — 3′ |
|---|---|---|
| −69 — −88 | Forward | GCGAGCTCGGTCCCACCCAG |
| −69 — −88 | Reverse | CTGGGTGGGACCGAGCTCGC |
| −49 — −68 | Forward | CAGGCGGGGGCGGGGCAGGG |
| −49 — −68 | Reverse | CCCTGCCCCGCCCCCGCCTG |
| −28 — −48 | Forward | CAGAGTGCTCCCCCCGCCCCC |
| −28 — −48 | Reverse | GGGGGCGGGGGGAGCACTCTG |
| −1 — −27 | Forward | CGCTTCCTCCCCGAGGGCCCCGGAGCC |
| −1 — −27 | Reverse | GGCTCCGGGGCCCTCGGGGAGGAAGCG |
| +22 — +1 | Forward | CAGCTCAGCCTCAGTCCCCGCA |
| +22 — +1 | Reverse | TGCGGGGACTGAGGCTGAGCTG |
| +40 — +23 | Forward | GCTTGTGCGGCGGCGTCG |
| +40 — +23 | Reverse | CGACGCCGCCGCACAAGC |
Pairs of complementary oligonucleotides used to create the double-stranded probes employed in the EMSA. In each case, the forward probe was biotinylated at the 3′ end before annealing.