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. Author manuscript; available in PMC: 2014 Aug 1.
Published in final edited form as: Am J Transplant. 2013 May 1;13(8):2169–2178. doi: 10.1111/ajt.12248

Table 1.

Recovery of cynomolgus monkey Treg from various sources by cell sorting

Treg source Absolute cell # (x106) Percentage of total cells
Fresh PBMC (n=3) 0.12±0.06 0.13±0.05
Cryopreserved PBMC (n=3) 0.19±0.14 0.23±0.11
Cryopreserved LN cells (n=4) 1.20±0.66 1.05±0.62

Absolute numbers of cynomolgus monkey Treg (CD4+CD25hiCD127−), as well as Treg percentages obtained by flow sorting from a single blood draw (cryopreserved or fresh PBMC) were generally low (0.13–0.23% of total cells) compared to those obtained from LN (Treg: 1% of total cells). Values are means ±1SD (n=10 experiments).