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. Author manuscript; available in PMC: 2014 Feb 1.

Published in final edited form as: Arterioscler Thromb Vasc Biol. 2012 Nov 21;33(2):257–265. doi: 10.1161/ATVBAHA.112.300200

Fig1 — (A) Significantly differentially expressed miRs in E2-treated mouse aortas. Relative quantification of miR expression was calculated by the 2^−ΔΔCt method. Data normalization was done using snoRNA202 as the endogenous control and ath-miR159a as the negative control. Results were shown as mean fold change (MFC) of two independent experiments. (B) Validation of E2-regulated miRs in mouse aorta. 10 miRs were selected from microarray data for qRT-PCR validation. Data were normalized with the vehicle-treated controls. Results were shown as mean ± S.D. of four independent experiments. *P<0.05, **P<0.01, refer to a comparison between E2- and vehicle-treated samples for qRT-PCR.

Fig1 — (A) Significantly differentially expressed miRs in E2-treated mouse aortas. Relative quantification of miR expression was calculated by the 2^−ΔΔCt method. Data normalization was done using snoRNA202 as the endogenous control and ath-miR159a as the negative control. Results were shown as mean fold change (MFC) of two independent experiments. (B) Validation of E2-regulated miRs in mouse aorta. 10 miRs were selected from microarray data for qRT-PCR validation. Data were normalized with the vehicle-treated controls. Results were shown as mean ± S.D. of four independent experiments. *P<0.05, **P<0.01, refer to a comparison between E2- and vehicle-treated samples for qRT-PCR.