Figure 9.

Increased pro-caspase-8 and caspase-8 levels were present at 48 hr post injury in CRI. Homogenate of whole ileum was analyzed for levels of pro-caspase-8 at 24 (a), 48 (b), and 72 hr (c). Similarly, caspase-8 was measured at 24 (g), 48 (h), and 72 hr (i) post-injury. Densitometry at 24, 48 and 72 hr is expressed as Procaspase-8 (Normalized) (d-f) and Caspase-8 (Normalized) (j-l). Procaspase-8 was 15 fold higher relative to sham at 48 hr and remained elevated relative to sham and radiation alone at 72 hr. Caspase-8 levels were double those of sham in burn alone at 24 hr and three times higher in CRI relative to sham at 48 hr post injury. All injury groups returned to sham levels by 72 hr. *p<0.05 vs. SHAM, #p<0.05 vs. BURN, $p<0.05 vs. RAD. Images are representative of 3 experiments, n=8-12 animals per injury group at each time point.