Table 2.
Cytotoxicity assay using splenocytes from BALB/c mice treated with extracts of E. uniflora and E. malaccensis.
| Extract | Concentrations (μg/mL) | |||||
|---|---|---|---|---|---|---|
| 1 | 5 | 10 | 25 | 50 | 100 | |
| E. uniflora | ||||||
| LEtOAc Fr. | − | − | − | − | + | + |
| Ln-BuOH Fr. | − | − | − | − | − | + |
| LH2O Fr. | − | − | − | − | − | − |
| SEtOAc Fr. | − | − | − | + | + | + |
| Sn-BuOH Fr. | − | − | − | − | − | + |
| SH2O Fr. | − | − | − | − | − | − |
| E. malaccensis | ||||||
| LEtOAc Fr. | − | − | − | − | + | + |
| Ln-BuOH Fr. | − | − | − | − | + | + |
| LH2O Fr. | − | − | − | − | − | + |
| SEtOAc Fr. | − | − | − | + | + | + |
| Sn-BuOH Fr. | − | − | − | − | − | + |
| SH2O Fr. | − | − | − | − | − | − |
| Saponin** | + | + | + | + | + | + |
Legend: *Nontoxic concentrations were determined by 3H-thymidine incorporation. Percentages below 30% compared to the immune cells not treated (control) are considered nontoxic (−). (LEtOAc Fr.): Ethyl acetate fraction of leaves, (Ln-BuOH Fr.): butanol fraction of leaves, (LH2O Fr.): aqueous fraction of leaves, (SEtOAc Fr.): ethyl acetate fraction of seeds, (Sn-BuOH Fr.): butanol fraction of seeds, and (SH2O Fr.): aqueous fraction of seeds.