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. Author manuscript; available in PMC: 2014 Sep 10.

Published in final edited form as: J Biotechnol. 2013 Jul 5;167(3):241–247. doi: 10.1016/j.jbiotec.2013.06.018

Optimization of conditions for conversion of NSNAH to NSNA2SHp using immobilized 2-OST, C5-epi and AST-IV. A. Effect of different substrate concentrations on conversion of NSNAH to NSNA2SHp: 0.1 mg/ml (); 1.0 mg/ml (); 10 mg/ml (). B. Effect of amount of PAPS on conversion of NSNAH to NSNA2SHp: 40 μM (); 200 μM (). C. Effect of different methods of reaction on conversion of NSNAH to NSNA2SHp: over 72 h and separate treatment of C5-epi (); normal treatment (). D. Effect of separate epimerization reaction on conversion of NSNAH to NSNA2SHp: three-step treatment (); normal treatment (). E. Effect of types of different reactors on the conversion of NSNAH to NSNA2SHp: column reactor (); batch reactor (). F. Effect of amount of enzymes on conversion of NSNAH to NSNA2SHp: 150 μg/ml (); 1500 μg/ml (). Error bars associated with analytical method are shown.

Inline graphic — Optimization of conditions for conversion of NSNAH to NSNA2SHp using immobilized 2-OST, C5-epi and AST-IV. A. Effect of different substrate concentrations on conversion of NSNAH to NSNA2SHp: 0.1 mg/ml (); 1.0 mg/ml (); 10 mg/ml (). B. Effect of amount of PAPS on conversion of NSNAH to NSNA2SHp: 40 μM (); 200 μM (). C. Effect of different methods of reaction on conversion of NSNAH to NSNA2SHp: over 72 h and separate treatment of C5-epi (); normal treatment (). D. Effect of separate epimerization reaction on conversion of NSNAH to NSNA2SHp: three-step treatment (); normal treatment (). E. Effect of types of different reactors on the conversion of NSNAH to NSNA2SHp: column reactor (); batch reactor (). F. Effect of amount of enzymes on conversion of NSNAH to NSNA2SHp: 150 μg/ml (); 1500 μg/ml (). Error bars associated with analytical method are shown.