Fig 3. Diagrams of rabbit IGKV and IGKC regions.

(a) Positions of IGKC1 and IGKC2 relative to IGKV genes. Arrows indicate transcriptional orientations as summarized in Table 4. The dashed line indicates the region between positions 98254316 and 98905144 (Table 4) where a few Vκ are present on the forward strand (+) and most are present on the reverse strand (−).

(b) Diagram of part of the IGKV and IGKJ regions associated with the IGKC1 region sequenced by Ros et al. (2005). GenBank Accession numbers are shown above the BAC clones. A total of 36 Vk were identified. The rabbit donor of DNA used for generating overlapping BAC clones 19602 (AY495828) and 215M22 (AY495826) was homozygous for the IGKC1 gene encoding the b5 allotype, as was the OryCun2.0 donor rabbit. BAC clone 179L1 (AY495827) from the LBLN-1 White Rabbit BAC library with IGKC1 that encoded the b4 allotype, includes DNA for two Vκ that overlapped BAC 215M22, and 5 additional Vκ as well as the (IGKJ1) region having five distinct J segments and IGKC1 of b4 allotype. Modified from Figure 1 in Ros F, Reichenberger N, Dragecic T, van Schooten W, Buelow R, Platzer J. (2005) Sequence analysis of 0.4 megabases of the rabbit germline immunoglobulin kappa1 light chain locus. Anim Genet 36:51-57, John Wiley & Sons Ltd and reproduced with permission of the publisher.