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. Author manuscript; available in PMC: 2013 Sep 23.
Published in final edited form as: Methods Mol Biol. 2012;814:499–514. doi: 10.1007/978-1-61779-452-0_33

Fig. 6.

Fig. 6

Glial-evoked dilation of retinal arteriole. (a) Confocal image of the retina, showing OGB-labeled glial cells and an arteriole (a) labeled with dextran rhodamine B isothiocyanate. The luminal diameter of the arteriole is monitored with confocal line scans (the white line across the vessel). Asterisk indicates the site of caged Ca2+ photolysis; scale bar, 25 μm. (b) Glial Ca2+ fluorescence measured near the arteriole. Photolysis of caged Ca2+ evokes a glial Ca2+ increase. (c) The luminal diameter of the arteriole. Photolysis evokes a transient increase in vessel diameter.