Fig. 4.

(A) Wild-type, loz1-1, and loz1Δ cells were grown in ZL-EMM supplemented with 0, 50, 200, or 500 μM zinc, cells were harvested, and total RNA was purified for RNA blot analysis. (B) Wild-type, loz1-1, and loz1Δ cells containing the loz1-lacZ reporter were grown in ZL-EMM supplemented with 0, 50, 200, or 500 μM zinc, and cells were harvested for β-galactosidase assays. (C) Wild-type and Loz1-myc cells were grown in ZL-EMM supplemented with 0 or 200 μM zinc, and total RNA was purified for RNA blot analysis. RNA blots were probed for adh4 and pgk1 mRNAs. (D) Wild-type and Loz1-myc cells were grown in ZL-EMM supplemented with 0, 50, 200, or 500 μM zinc, and crude protein extracts were isolated for immunoblot analysis. (E) loz1Δ cells expressing pLoz1-GFP were grown in ZL-EMM supplemented with 0 (-Zn) or 200 μM (+Zn) zinc. The cells were visualized for GFP by fluorescence microscopy. Hoechst stain was used to stain the nucleus, and differential interference contrast microscopy was used to confirm cell integrity.