Fig. 1.

The JAZ10-GusPlus (JGP) reporter for JA-mediated wound signaling in 5-d.o. WT and aos mutant Arabidopsis seedlings. Detection of GUS activity was performed 2 h after wounding. Red arrows indicate cotyledon wounding sites. The reporter in the aos mutant background displays a constitutive faint blue staining in the aerial organs not observed in any other genotype and not attributable to a higher basal JAZ10 transcription (SI Appendix, Fig. S2). (Inset) Close-up of root. (Scale bars, 0.5 mm.)