Fig. 3.
ninja mutants display constitutive JAZ10 expression. (A) JGP expression in control and wounded seedlings of ninja-1 and ninja-1 aos (compare with WT and aos in Fig. 1). Arrows indicate constitutive reporter activity in hypocotyl and roots (black) and cotyledon wounding sites (red). (Inset) Close-up of root. The ninja-2 aos double mutant displays the same expression pattern as ninja-1 aos. (Scale bars, 0.5 mm.) (B and C) qRT-PCR of JAZ10 expression basally (B) and 1 h after wounding (C) in ninja-1 (n-1), ninja-2 (n-2), ninja-3 (n-3), ninja RNAi (ni), jin1-7 (j1), ninja-1 jin1-7 (n-1 j1), and ninja-3 jin1-7 (n-3 j1). JAZ10 transcript levels were normalized to those of UBC21 and displayed relative to the expression of unwounded WT controls (B) or wounded WT samples (C); thus, WT levels are set to 1 in each plot and indicated with a dashed line. Bars represent the means of three biological replicates (±SD), except for n-2 and ni (two biological replicates), each containing a pool of organs from ∼60 seedlings. Complete qRT-PCR data are in Dataset S1.