Fig. 1.

Generation and characterization of a Cre-activated TRAP allele. (A) TRAP strategy to pull down transcripts bound to GFP-tagged ribosomes. (B) Structure of targeted Rosa26^fsTRAP allele. (C) EGFP-L10a incorporates into assembled ribosomes and polysomes. Equal volumes of the indicated fractions (1–4) from sucrose gradient polysome fractionation of Rosa26^CAG-TRAP ES cells were assayed for GFP-L10a by Western blot. (D) Section of E12.5 TNT-Cre::Rosa26^fsTRAP heart. TNNI3-positive cardiomyocytes coexpressed GFP. (E) Western blot showing Cre-dependent expression of GFP-L10a fusion protein in neonatal Rosa26^fsTRAP heart. (F) Germ-line Cre recombination yielded Rosa26^CAG-TRAP mice, which showed widespread GFP-L10a expression.