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. 2013 Sep 3;110(38):15395–15400. doi: 10.1073/pnas.1304124110

Fig. 2.

Fig. 2.

Capture of endothelial transcripts from heart with Tie2-TRAP. (A) Tie2-Cre–activated Rosa26fsTRAP expression in endothelial cells. Boxed area at Left is magnified at Right. LV, left ventricle. (B) Transcript enrichment by qRT-PCR in Tie2-TRAP or TNT-TRAP isolated from heart. (C) Heatmap showing an overview of genes differentially expressed between Tie2-TRAP and TNT-TRAP of heart RNA by RNA-seq. (D) Histogram of Tie2-TRAP:input ratios, showing high values (>5) for known endothelial-restricted transcripts. (E) qRT-PCR measurement of Tie2-TRAP:input ratio in heart RNA of arbitrarily selected genes with an RNA-seq ratio greater than 5. Tnnt2 and Myh6 were included as negative controls. (F) Eurexpress high-throughput in situ hybridization images of selected genes with high Tie2-TRAP:input ratio. The classic endothelial gene Tek (also known as Tie2) has a typical endothelial/endocardial staining pattern. Prkd2 and Tspan6 are less-characterized genes with endothelial-restricted expression identified by high Tie2-TRAP:input ratios. See also Fig. S2. Data in B and E are displayed as mean ± SEM.