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A. Relative phosphotriesterase activities of W263 saturation site variants are screened with 1 mM of paraoxon substrate. B. Relative lactonase activity of W263 saturation site variants screened for 3-oxo-C12 AHL hydrolysis (see methods for more details). C. Catalytic efficiency comparisons between best selected variants. For screening experiments (A-B.), partially purified enzymes (> 70%) were used.
