Table 3. Phosphotriesterase activity comparison between wild-type and W263F SsoPox.
|
Substrate
|
SsoPox wt*
|
SsoPox W263F
|
|||||
|---|---|---|---|---|---|---|---|
| Name | Additive | kcat (s-1) | kM (µM) | kcat/kM (s -1 M-1) | kcat (s-1) | kM (µM) | kcat/kM (s -1 M-1) |
| Paraoxon (I) | - | 12.59 ± 1.26 | 24 250 ± 3 716 | 5.19 (±0.95)x102 | 8.47 ± 0.53 | 700 ± 146 | 1.21 (±0.26)x104 |
| + SDS 0.1% | 40.72 ± 7.70 | 12 340 ± 3 625 | 3.30 (±1.15)x103 | 117.7 ± 6.0 | 2 462 ± 302 | 4.78 (±0.64)x104 | |
| + SDS 0.01% | 24.59 ± 1.77 | 3 832 ± 626 | 6.42 (±1.15)x103 | 85.85 ± 4.85 | 1 168 ± 194 | 7.35 (±1.29)x104 | |
| CMP-coumarin (II) | - | ND | ND | 8.13 (±0.08)x103 | 9.41 ± 0.38 | 114.3 ± 13.7 | 8.23 (±1.04)x104 |
| + SDS 0.01% | 25.47 ± 0.42 | 137.0 ± 7.0 | 1.86 (±0.10)x105 | 8.64 ± 0.39 | 60.0 ± 9.5 | 1.44 (±0.24)x105 | |
| IMP-coumarin (III) | - | ND | ND | 1.67 (±0.04)x103 | 8.39 ± 1.63 | 94.8 ± 26.2 | 8.85 (±2.99)x104 |
| PinP-coumarin (IV) | - | ND | ND | ND | 0.11 ±0.02 | 16.1 ± 5.49 | 7.08 (±2.58)x103 |
Roman numbers indicate the chemical structures of indicated molecules presented in Figure S1 . * Catalytic parameters are from Hiblot et al. (2012). ND corresponds to an undetermined value. When Vmax could not be reached, the linear part of the MM plot was fitted to a linear regression and correspond to the catalytic efficiency.