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. 2013 Sep 23;8(9):e75210. doi: 10.1371/journal.pone.0075210

Table 4. Dead end metabolites remaining in EcoCyc.

Dead end metabolite EcoCyc reaction Enzyme
(E)-3-(methoxycarbonyl)pent-2-enedioate trans-aconitate + S-adenosyl-L-methionine → (E)-3-(methoxycarbonyl)pent-2-enedioate + S-adenosyl-L-homocysteine trans-aconitate methyltransferase (tam)
2-aminobutyrate an aminated amine donor + 2-oxobutanoate → 2-aminobutyrate + a deaminated amine donor valine-pyruvate aminotransferase (avtA)
2-aminomalonate-semialdehyde L-serine + NADP+ → 2-aminomalonate-semialdehyde + NADPH + 2H+ 3-hydroxy acid dehydrogenase (ydfG)
2-deoxygluconate; 3-dehydro-2-deoxy-D-gluconate NAD+ + 2-deoxygluconate → NADH + 3-dehydro-2-deoxy-D-gluconate + H+ 2-deoxy-D-gluconate 3-dehydrogenase (kduD)
3-methylcrotonyl-CoA; isovaleryl-CoA isovaleryl-CoA + an oxidized electron-transfer flavoprotein → 3-methylcrotonyl-CoA + a reduced electron-transfer flavoprotein isovaleryl-CoA dehydrogenase (aidB)
3-sulfinoalanine 3-sulfinoalanine + H2O → L-alanine + sulfite + H+ L-cysteine desulfurase (sufS) / cysteine sulfinate desulfinase (csdA)
3-α,12-α-dihydroxy-7-oxo-5-β-cholanate cholate + NAD+ → 3α,12α-dihydroxy-7-oxo-5β-cholan-24-oate + NADH + H+ 7-α-hydroxysteroid dehydrogenase (hdhA)
4-hydroxy-2-oxoglutarate 4-hydroxy-2-oxoglutarate ← glyoxylate + pyruvate multifunctional 2-keto-3-deoxygluconate 6-phosphate aldolase (eda)
aminoacetaldehyde taurine + 2-oxoglutarate + oxygen → aminoacetaldehyde + sulfite + succinate + CO2 + H+ taurine dioxygenase (tauD)
curcumin curcumin + NADPH + H+ → dihydrocurcumin + NADP+ NADPH-dependent curcumin/dihydrocurcumin reductase (curA)
tetrahydrocurcumin dihydrocurcumin + NADPH + H+ → tetrahydrocurcumin + NADP+ NADPH-dependent curcumin/dihydrocurcumin reductase (curA)
diacetyl (S)-2-acetolactate + an oxidized electron acceptor + H+ → diacetyl + CO2 + a reduced electron acceptor acetoin + NADP+ → diacetyl + NADPH + H+ yohF (predicted oxidoreductase)
ethyl-(2R)-methyl-(3S)-hydroxybutanoate; ethyl-2-methylacetoacetate ethyl-(2R)-methyl-(3S)-hydroxybutanoate + NADP+ → ethyl-2-methylacetoacetate + NADPH + H+ β-keto ester reductase (dkgA)
GDP-α-D-glucose GDP-α-D-glucose + H2O → β-D-glucose + GDP + H+ GDP-mannose mannosyl hydrolase (gmm)
hydroxylamine pyruvate + hydroxylamine → pyruvic oxime + H2O hydroxylamine + a reduced electron acceptor → ammonia + an oxidized electron acceptor + H2O hybrid-cluster protein (hcp)
linear dimeric GMP cyclic di-3',5'-guanylate + H2O → linear dimeric GMP + H+ cyclic di-GMP phosphodiesterase
L-rhamnonate L-rhamnonate → 2-keto-3-deoxy-L-rhamnonate + H2O L-rhamnonate dehydratase (yfaW)
nicotinamide mononucleotide (reduced) (NMNH) NADH + H2O → NMNH + AMP + 2H+ NADH pyrophosphatase (nudC)
oxamate oxamate + carbamoyl-phosphate ← oxalurate + phosphate -
quinate NAD(P)+ + L-quinate → NAD(P)H + 3-dehydroquinate + H+ quinate dehydrogenase (ydiB)
S-adenosyl-4-methylthio-2-oxobutanoate S-adenosyl-L-methionine + 7-keto-8-aminopelargonate → S-adenosyl-4-methylthio-2-oxobutanoate + 7,8-diaminopelargonate 7,8-diaminopelargonic acid synthase (bioA)
tetrahydromonopterin tetrahydromonapterin + NADP+ ← 7,8-dihydromonapterin + NADPH + H+ dihydromonapterin reductase (folM)
urate xanthine + NAD+ + H2O → urate + NADH + H+ xanthine dehydrogenase (xdhABC)