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. 2013 Sep 23;8(9):e75331. doi: 10.1371/journal.pone.0075331

Figure 4. Detection of NO produced in explanted murine carotid arteries ex vivo using Cu 2FL2E (20 µM) after precontraction.

Figure 4

(a) Detection of NO in response to NA (ECs and SMCs are not apparent), (b) Detection of NO in post NA and ACh stimulation (2.5min) (ECs and SMCs are apparent), (c) Syto 41 staining of nucleus of ECs and SMCs, (d) plot of fluorescence intensities of the ECs and SMCs (from carotid artery) measured with NA and ACh stimulation for 15min.