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. 2013 Sep 23;8(9):e75188. doi: 10.1371/journal.pone.0075188

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© 2013 Ye et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Purification of NS5-interacting proteins using the TAP method. Cell lysates from 293T cells transfected with either the pcD-Flag-HA-NS5 DNA (lane 1), or pcD-Flag-HA vector (lane 2) were purified using the TAP procedure as described under “Experimental Procedures.” The purified proteins were visualized by silver staining. The protein bands in lane 1 which is different with that in lane 2 were excised and analyzed using LC-MS/MS. The position and names of proteins identified by MS are indicated. (B) Western blot analysis of the purified proteins. Anti-Flag antibody was used to detect Flag-HA-NS5 protein. In lane 1 of both panels, the black arrowheads mark the expected full length Flag-HA-NS5.