Figure 6. Minocycline treatment potentiates NF-κB activation in gp91^phox-/- 6-OHDA lesioned mice.

In A, representative panel of NF-κB activation in striatal nuclear extracts. In B, densitometric analysis of NF-κB bands obtained from nuclear extracts of striatum. In C, representative panel of NF-κB activation in SN nuclear extracts. In D, densitometric analysis of the NF-κB bands obtained from nuclear extracts of SN. The statistical significance is expressed as: * P<0.05; ** P<0.01 and *** P<0.001; n=4. Competition studies were performed using brain extract (15 µg) in the absence or presence of unlabeled specific (NF-κB consensus sequence, 5-, 10- and 20- fold molar excess) or non-specific oligonucleotide (TFIID consensus sequence, 20- fold molar excess), as indicated. The position of specific NF-κB/DNA binding complexes is indicated (NF-κB). NS represents no specific binding (NS). The localization of the probe is also indicated. Results are representative of three experiments.