Figure 1. Inhibition of PKD isoforms by 1-NA-PP1 and IKK-16.

Inhibition of recombinant human PKD1, 2 and 3 was assayed in the presence of 10 different concentrations of 1-NA-PP1 (A) and IKK-16 (B) by an in vitro radiometric PKD kinase assay. The IC₅₀ values were calculated as the mean ±SEM of at least three independent experiments with triplicate determinations at each concentration of drug in each experiment. The data were plotted as a function of drug concentration and a representative graph is shown.