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. 2013 Sep 23;8(9):e75497. doi: 10.1371/journal.pone.0075497

Figure 5. Structurally diverse LRP1 ligands such as MMP-9-PEX, but not mutated RBD (muRBD), promoted sensory neurite extensions.

Figure 5

A, Phase contrast images of cultured sensory neurons treated with the hemopexin domain of MMP-9 (MMP-9-PEX; 100 nM), mutated RBD (muRBD; lysine residues 1370 and 1374 that are essential for LRP1 binding are replaced by alanine; 100 nM) or GST (100 nM). Scale bar, 50 µm. B, Immunofluorescence microscopy of β-neuronal class III tubulin (Tuj1; green) in primary DRG neurons cultured for 2 weeks in MMP-9-PEX or GST. Scale bar, 10 µm. Cell nuclei are identified by Dapi (blue). Images represent n=2 independent replicates. C, GAP-43 mRNA levels in cultured sensory neurons treated with GST, MMP-9-PEX or muRBD every other day for 2 weeks. Results are compared to vehicle-treated control cultures. Data are expressed as mean±SEM (n=2-4). **p < 0.01 compared with controls.