Table 2. Effect of huprines pretreatment on NGF differentiated PC12 cells survival after exposure of cells to H₂O₂ (200 µM).

TREATMENT	% PROTECTION 48 H
Huprine X (1 µM)	53±5.0*
Huprine X (0.1 µM)	44±6.0*
Huprine X (1 µM) +MEC (100 µM)	7±3.2b
Huprine X (1 µM) +ATR (0.1 µM)	32±2.7b
Huprine Y (1 µM)	56±5.0*
Huprine Y (0.1 µM)	49±6.1*
Huprine Y (1 µM) +MEC (100 µM)	2.3±0.9b
Huprine Y (1 µM) +ATR (0.1 µM)	16±1.6b
Huprine Z (1 µM)	56±7.0*
Huprine Z (0.1 µM)	47±7.5*
Huprine Z (1 µM) +MEC (100 µM)	17±3.4b
Huprine Z (1 µM) +ATR (0.1 µM)	27±7.1b

Effect of antagonists mecamylamine (MEC) and atropine (ATR) on huprines protective effect on PC12 cells survival after exposure of the cells to H₂O₂. See Methods for details of cell treatment and conditions. Values are expressed as percentage of protection (mean ± s.e.m.) obtained from at least three independent experiments run in triplicate. *P<0.05 as compared with H₂O₂–treated group; ^bP<0.05 as compared with 1 µM concentration of corresponding drug (Dunnett's test).