Figure 3. Effect of VDR transfection on difference between RUNX2 17A and 11A constructs in transfection.

Constructs were transfected at four concentrations (1, 15, 30 and 45 ng) with or without VDR (10 ng) and with or without treatment with 1,25(OH)₂D₃ (1,25D3 in figure) at 10⁻⁷ M. Data presented are the marginal means derived from the general linear model and adjusted by linear regression to 12.5 ng of transfected vector. Error bars are one standard error of the mean. Promoter activity is the control Renilla adjusted pOSLUC firefly luciferase activity. In all cases within a treatment, is the comparison between RUNX2 17A and 11A significantly different excepting the single case marked where cells were transfected with VDR and treated with 1,25(OH)₂D₃.