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. 2013 Sep 23;8(9):e74029. doi: 10.1371/journal.pone.0074029

Figure 6. SN stimulates proliferation and reduces apoptosis of HUVECs under hyperglycemic conditions.

Figure 6

(A) HUVECs were cultured with medium containing mannitol or glucose and stimulated with SN 10 ng/ml, SN 100 ng/ml and VEGF 50 ng/ml. Proliferation was determined using a BrdU assay. Proliferation was stimulated by SN and VEGF to a comparable extent even under hyperglycemic conditions. *, p<0.001 vs. mannitol or glucose respectively. (B) Apoptotic cells were identified as TUNEL positive cells and are shown as percentage of the total cell number (obtained by DAPI staining). SN and VEGF significantly inhibited apoptotic reactions even under hyperglycemic conditions. *, p<0.001; #, p<0.05; §, p<0.005 vs. mannitol or glucose respectively.