Figure 3. Doxorubicin-induced gene expression in non-selected and doxorubicin-selected FL/ΔAkt-1:ER*(Myr+) + ΔRaf-1:AR cells. The effects of different concentrations of doxorubicin on MAPK, Akt, p53 and Bcl-XL expression were examined in non-selected and doxorubicin-selected FL/ΔAkt-1:ER*(Myr+) + ΔRaf-1:AR cells after they were cultured in the presence of 4HT + Test for 24 h in the absence of doxorubicin. Cells were then stimulated with the indicated concentrations of doxorubicin for 4 h and then western blot analysis was performed.