Table 3.
Total activity (U) | Total protein (mg) | Specific activity (U/mg) | Purification (fold) | Yield (%) | |
---|---|---|---|---|---|
Culture filtrate | 1,264.05 | 28.33 | 44.61 | 1 | 100 |
Ammonium sulfate precipitation | 850.19 | 10.93 | 77.761 | 1.74 | 67.25 |
Dialysis | 611.92 | 7.08 | 86.38 | 1.93 | 48.41 |
Sephadex G-100 (gel filteration) | 339.21 | 0.86 | 391.08 | 5.02 | 39.89 |