Fig. 4.

Effect of enhanced rapamycin-induced autophagy by overexpression of Beclin 1. Beclin 1 was overexpressed in HT29 cell by infection. HT29 cell was infected with virus containing Beclin 1-HA for 72 h and detected by immunoblotting with antibody against HA (a). Rapamycin-induced autophagy was enhanced of by infection of Beclin 1. HT29 cell was infected with Beclin 1 or mock, and treated with 100 nM rapamycine for 24 h. Cells were lysed and processed for immunoblotting with antibody against LC3 (b). LC3II/Actin was quantified (c). Data were presented as a mean ± SD of three independent experiments. **P < 0.01. Overexpression of Beclin 1 reduces growth rate in HT29 cells and enhances the rapamycin-induced anti-tumor effect on HT29 cells (d). ** Beclin 1 versus Beclin 1 + Rapa P < 0.01