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. 2013 Sep 24;7:163. doi: 10.3389/fncel.2013.00163

FIGURE 2.

FIGURE 2

Target protectors for Pten block miR-19a-induced repression. (A) Binding sites of miR-19a in the Pten 3′UTR and complementary target protector sequences for the second miR-19a binding site. The seed binding sequence of miR-19a is highlighted in green, and the entire length of miR-19a along the Pten 3′UTR is highlighted in red. (B) Luciferase assays of target protector effects on miR-19a repression of the second miR-19a binding site in the Pten 3′UTR. miR-19a reduced luciferase activity in the absence of target protector. The 60 nt target protector but not the 22 or 40 nt target protector recovered luciferase activity of the Pten 3′UTR. Neither miR-19a nor the 60 nt target protector had an effect on the luciferase activity of the full length Pten 3′UTR when the miR-19a binding sites were mutated. Data are presented as mean ± SEM; n = 3 luciferase assays; p values in relation to control (***p < 0.01). n.s., not significant.