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. 2013 Sep 24;7:163. doi: 10.3389/fncel.2013.00163

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Copyright © Knauss, Bian and Sun.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Target protectors are transcribed and effective in vitro. (A) Schematic vector map of the 60 nt target protector in pcDNA3.1. (B) The 60 nt target protector and control target protector DNA were detected by Northern blot assays at the expected size. No target protector was detected in RNA extracted from empty vector-transfected cells. (C,D) The endogenous protein levels of Pten were decreased upon transfection of miR-19a but recovered upon cotransfection of miR-19a and the 60 nt target protector. Transfection of the 60 nt target protector alone also showed an increase in Pten. Data are presented as mean ± SD; n = 3 separate protein extractions; p values in relation to control (***p < 0.001).