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. 2013 Sep 24;7:163. doi: 10.3389/fncel.2013.00163

FIGURE 5.

FIGURE 5

miR-19a targeting of Pten is responsible for RGC expansion in the developing mouse cortex. (A,B) miR-19a targeting of Pten is important for RGC expansion. Introduction of the 60 nt target protector at E13.5 for analysis at E14.5 significantly decreased the number of RGCs colabeled with GFP and Pax6. Ectopic expression of Pten-FL-3′UTR showed a similar effect, while co-electroporation of Pten-FL-3′UTR and miR-19a ablated this effect. (C,D) Ectopic expression of the 60 nt target protector, Pten-FL-3′UTR, or Pten-FL-3′UTR and miR-19a had no effect on the number of IPs colabeled with GFP and Tbr2. Data are presented as mean ± SEM; n ≥ 3 for all electroporations; p values in relation to control (***p < 0.001). n.s., not significant. Scale bar: 50 μm.