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. 2013 Sep 24;4:188. doi: 10.3389/fgene.2013.00188

Figure 1.

Figure 1

ER stress causes substantial hepatic lipid accumulation within 8 h. (A) C57BL/6J mice were challenged with 1 mg/kg TM for the indicated times, livers were frozen in OCT, and lipids were stained with Oil Red O. Scale bar = 50 μm. (B) Same as (A), except lipid content was assessed by immunohistochemical staining for the lipid droplet marker protein ADRP. Scale bar = 50 μm. (C) Same as (A), except triglyceride content was measured by colorimetric assay after extraction of neutral lipids. p < 0.001 by One-Way ANOVA. n = 3 samples per time point. Error bars here and elsewhere show means ± SDM.