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. 2013 Sep 24;6:26. doi: 10.3389/fnmol.2013.00026

Figure 1.

Figure 1

Screening of FlincG variants. (A) Schematic diagram illustrating the general FlincG design and specifying the modifications tested. The diagram also defines the nomenclature adopted in text. (B) Brightfield (top) and basal fluorescence (bottom) images of HEKGC/PDE5 cells 3 days after attempted transfection with identical amounts (0.5 μg/well) of AdV-FlincG plasmid DNA (left) and FGB DNA (right). Scale bar = 100 μm. (C) Sample FACS spectra of suspensions of HEKGC/PDE5 cells transfected with the indicated FlincG variants, before (black) and after (red) incubation with 8-bromo-cGMP (1 mM). Calibration bars: vertical = frequency; horizontal = log intensity (arbitrary units). Arrows and arrowheads indicate untransfected and transfected components of the control spectra, respectively. (D–F) Results of FACS analysis for groups of FlincG variants; plasmids in panel D all bore a strong Kozak consensus sequence (see Material and Methods). Numbers in parentheses are n-values.